Flavia Souza, PhD, MS
Ph.D. in Physiological Sciences, UFES, Brazil / LSUHSC, New Orleans
M.S. in Physiological Sciences, UFES, Brazil
B.S. in Physical Therapy, EMESCAM, Brazil
My postdoctoral training focus has been the impact of alcohol on molecular signals controlling intrinsic lymphatic pumping. I started in 2009 at the Physiology Department at LSUHSC, in Dr. Jerome W Breslin and Dr. Patricia Molina’s laboratories. Building upon the techniques I learned as a graduate student, I have adapted those skills and mastered techniques such as mesenteric lymphatic and venule isolation and cannulation, structured-illumination microscopy, venule permeability protocol, intragastric catheter implementation surgery, and calcium imaging. Based on other previously published reports combined with my techniques I developed a ratiometric imaging of fura-2 calcium imaging for lymphatic vessels. I also optimized techniques such as isolated lymphatic labeling for immunofluorescence using a chamber built by our laboratory. In summary, I mastered survival animal surgery, lymphatic isolation protocols along with analyses. More recently I have developed a novel area of research interest on the role of lymphatic vessels as a route for gut-derived lipopolysaccharide (LPS) transport following repeated binge-like alcohol intoxication. I am particularly interested in the interaction between molecules transported and leaked from mesenteric lymphatic vessels into perilymphatic adipose tissue (PLAT). Our working hypothesis is that this lymphatic-PLAT crosstalk contributes to development of insulin resistance resulting from alcohol abuse. My research is funded by an individual NRSA award and LSU Department of Physiology funds.
Souza-Smith FM, Kurtz KM, Molina PE, Breslin JW. (2010). Adaptation of Mesenteric Collecting Lymphatic Pump Function Following Acute Alcohol Intoxication. Microcirculation, 17, 514-524.
Katz PS, Trask AJ, Souza-Smith FM, Hutchinson KR, Galantowicz ML, Lord KC, Stewart JA Jr, Cismowski MJ, Varner KJ, Lucchesi PA.Coronary arterioles in type 2 diabetic (db/db) mice undergo a distinct pattern of remodeling associated with decreased vessel stiffness. Basic Res Cardiol. 2011, Jul 10. PMID: 21744279
Souza-Smith FM, Katz PS, Trask AJ, Stewart JA, Lord KC, Varner KJ, Vassallo DV, Lucchesi PA. Mesenteric resistance arteries in type 2 diabetic mice undergo outward remodeling. PLoS One. 2011 Aug; 6(8):e23337. PMID: 21829729
Souza-Smith FM, Kurtz KM, Breslin JW. Measurement of cytosolic Ca2+ in isolated contractile lymphatics. J Vis Exp. 2011 Dec 8;(58). pii: 3438. doi: 10.3791/3438. PMID: 22214883